Russian Journal of Pediatric SurgeryRussian Journal of Pediatric SurgeryДетская хирургия1560-95102412-0677Eco-Vector67010.17816/ps670Original Study ArticlesОригинальные исследованияResearch ArticlePlatelet monoamine oxidase and erythrocyte adenosine triphosphatase as biomarkers of necrotizing enterocolitisМоноаминоксидаза тромбоцитов и аденозинтрифосфатазы эритроцитов как биомаркеры некротизирующего энтероколитаhttps://orcid.org/0000-0002-3745-36906298-8131VinelPolina K.ВинельПолина Константиновна
Russian Federation

MD

vinelpolina@icloud.comhttps://orcid.org/0000-0002-6695-73887966-9068TsarevaValentina V.ЦарёваВалентина Викторовна
Russian Federation

MD, Cand. Sci. (Medicine)

кандидат медицинских наук

semenovatsareva@mail.ruhttps://orcid.org/0009-0009-2627-0459BaboshkoPavel G.БабошкоПавел Григорьевич
Russian Federation
904306008@mail.ruhttps://orcid.org/0000-0001-5687-39763681-1816SinitskiiAnton I.СиницкийАнтон Иванович
Russian Federation

MD, Dr. Sci. (Medicine), Associate Professor

доктор медицинских наук, доцент

Sinitskiyai@yandex.ruhttps://orcid.org/0009-0008-5400-3043KozhevnikovArtem S.КожевниковАртём Сергеевич
Russian Federation

MD

vk_523@mail.ruhttps://orcid.org/0009-0007-9327-7779GruninAlexandr V.ГрунинАлександр Васильевич
Russian Federation

MD

sasha_grunin@mail.ruSouth-Ural State Medical UniversityЮжно-Уральский государственный медицинский университетChelyabinsk Regional Pediatric HospitalЧелябинская областная детская клиническая больница17042024300520242821241321305202329032024Copyright ©; 2024, Vinel P.K., Tsareva V.V., Baboshko P.G., Sinitskii A.I., Kozhevnikov A.S., Grunin A.V.Copyright ©; 2024, Винель П.К., Царёва В.В., Бабошко П.Г., Синицкий А.И., Кожевников А.С., Грунин А.В.2024Vinel P.K., Tsareva V.V., Baboshko P.G., Sinitskii A.I., Kozhevnikov A.S., Grunin A.V.Винель П.К., Царёва В.В., Бабошко П.Г., Синицкий А.И., Кожевников А.С., Грунин А.В.https://jps-nmp.ru/jour/article/view/670

BACKGROUND: The diagnostics of necrotizing enterocolitis is a complex and often ineffective issue. Biomarkers reflecting key links in the pathogenesis of the disease may serve as early predictors of development, progression, and severe course of necrotizing enterocolitis.

AIM: To assess potentials of platelet monoamine oxidase activity and erythrocyte ATPase to predict the development of necrotizing enterocolitis in premature newborns.

METHODS: The present study had two stages and included 28 children who were treated at the Chelyabinsk Regional Children’s Clinical Hospital from November 2021 to December 2022. At the first stage, premature newborns were examined; among them a group of newborns with necrotizing enterocolitis IIB–IIIA-B was identified. Venous blood was used for testings. At the second stage, specimens obtained from the intestine during surgical interventions in newborns with surgical pathology were examined additionally as well.

RESULTS: Children with necrotizing enterocolitis showed a significant decrease in the specific activity of platelet monoamine oxidase which correlated with the activity of monoamine oxidase in gut specimens. The following changes were registered: decrease in the activity of magnesium adenosine triphosphatasein erythrocytes by 50–100% of the maximum enzyme activity in the control group; decrease of sodium-potassium adenosine triphosphatase activity y 4 times from the maximum values in the control group. There was also a significant increase in the activity of calcium adenosine triphosphatase activity in erythrocytes.

CONCLUSION: The obtained data allow to suggest that platelet monoamine oxidase in preterm newborns may potentially serve as an indicator of tissue and organ immaturity, rather than a marker of inflammation and oxidative damage. Changes in erythrocyte adenosine triphosphatase activity in preterm infants with surgical stages of necrotizing enterocolitis indicate a hypoxic hypoenergetic state, accompanied by high concentrations of intracellular calcium.

The obtained data are promising for developing new methods for diagnosis/prognosis of necrotizing enterocolitis in newborns.

Обоснование. Диагностика некротизирующего энтероколита сложна и часто малоэффективна. Ранними предикторами развития, прогрессирования и тяжёлого течения заболевания могут являться биомаркеры, отражающие ключевые звенья этиопатогенеза.

Цель. Оценка потенциальной возможности определения активности тромбоцитарной моноаминоксидазы и аденозинтрифосфатаз эритроцитов для прогнозирования развития некротизирующего энтероколита у недоношенных новорождённых.

Методы. Исследование проведено в два этапа и включало 28 детей, находившихся на лечении в ГАУЗ «Челябинская областная детская клиническая больница» в период с ноября 2021 г. по декабрь 2022 г. На первом этапе обследовали недоношенных новорождённых, среди которых набрана группа детей с некротизирующим энтероколитом IIБ–IIIА-Б стадии. Для исследования использовали венозную кровь. Второй этап включал дополнительное исследование препаратов кишки, полученных при проведении оперативного вмешательства у новорождённых с хирургической патологией.

Результаты. У детей с некротизирующим энтероколитом наблюдали достоверно значимое снижение специфической активности моноаминоксидазы тромбоцитов, коррелирующее с активностью моноаминоксидазы в препаратах кишки. Установили снижение активности магниевой аденозинтрифосфатазы эритроцитов на 50–100% от максимальной активности фермента в контрольной группе, снижение активности натрий-калиевой аденозинтрифосфатазы эритроцитов в 4 раза от максимальных значений группы контроля, значительное повышение активности кальциевой аденозинтрифосфатазы эритроцитов.

Заключение. Полученные данные позволяют предположить, что моноаминоксидаза тромбоцитов у недоношенных новорождённых потенциально может является индикатором незрелости тканей и органов, а не маркером воспаления и окислительного повреждения. Изменения активности аденозинтрифосфатаз эритроцитов у недоношенных с хирургическими стадиями некротизирующего энтероколита свидетельствуют о гипоксическом гипоэнергетическом состоянии, сопровождающимся высокими концентрациями внутриклеточного кальция. Результаты исследования перспективны с точки зрения разработки новых методов диагностики/прогноза некротизирующего энтероколита новорождённых.

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