REPARATION OF BONE TISSUE IN THE AREA OF OPEN FRACTURE CONSOLIDATION IN TUBULAR BONE IN LABORATORY ANIMALS. AN EXPERIMENTAL STUDY

Objective. Antibiotic prophylactics in surgery is a standard of medical care for many surgical interventions. It is “a very short course”: starts immediately before surgery and lasts no more than 24 hours after it. There were no any information found after a broad search on pathomorphology of consolidation zone in open fracture and morphometry of bone callus structures under various modalities of perioperative antibiotic prophylactics. Purpose. To find out reparation degree and inflammatory changes in bone tissue in the area of consolidation in open femoral fracture in experimental animals under various modalities of perioperative antibiotic prophylactics. Material and methods. 60 outbred male guinea pigs were taken into the experiment. The experimental animals were divided into 3 groups, 20 individuals in each. Animals from Group I, so-called “AB-1”, were given antibiotics 30 minutes before surgery and then for 8 hours after it during one day. Animals from Group II, so-called “AB-3” were administered antibacterial drug for 3 days of postoperative period. Guinea pigs from Group III (Controls) had no antibiotic therapy. The modeling of femur open fracture was performed according to the developed methodology for modeling and treatment of open fractures of tubular bones in experiment. Results. When comparing reparative processes in AB-1 and AB-3 groups, similar regeneration processes in femoral fracture were noted. They were accompanied by: formation of endostal and periosteal callus with abundantly vascularized connective tissue; formation of significant amount of newly formed bone trabeculae; insignificant presence of leukocytes in the lumen of bone marrow canal, what may be explained by peculiarities of postoperative management of laboratory animals. In the Control group, growth of newly formed bone trabeculae was less intensive ( less by 16.3%); there also were 2-fold increase in connective tissue volume and actively proliferating zones of cartilage tissue. Conclusion. Thus, findings of morphological examination indicate that in the experimental group with short 24-hour perioperative antibiotic prophylaxis, bone tissue consolidation had minimal inflammatory changes and better formation of new bone trabeculae.

antibiotic prophylactics, open fracture, morphology, consolidation