Acute systemic and subacute toxicity assessment of new alloy based on titanium nickelide with the addition of silver to replace bone defects in children



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Abstract

BACKGROUND: Currently, restoration of bone tissue damaged in case of injuries and surgical interventions remains an urgent problem in medicine. The combination of titanium nickelide (NT) with silver seems promising in terms of reducing the activity of the inflammatory process, speedy regeneration and restoration of damaged bone tissue function.

AIMS: to determine the possible acute systemic and subacute toxicity of an innovative titanium nickelide alloy with silver in an experiment on laboratory animals.

METHODS: the study of acute systemic toxicity was carried out in white outbred mice (17-23g). The supernatant liquid of NT alloy powder with the addition of 0.5% silver on a water basis was injected into the tail vein (n=10), on a non-aqueous basis - intraperitoneally (n=10), in a volume of 50 ml/kg. The corresponding medium was used as a control (n=10). To identify subacute toxicity, samples were sewn into the thigh muscles of Wistar rats (250-320g): three experimental groups (n=10) - NT with 0.3% silver, 0.5% silver and 0% silver. The fourth group (n=10) - control (wound modeling without powder injection). After 28 days, the animals were removed from the experiment, and hematological and biochemical blood tests were performed.

RESULTS: no animal deaths or adverse clinical signs were recorded following the acute systemic toxicity test. The number of erythrocytes in the blood of experimental animals after intramuscular administration of NT with the addition of 0.5% silver was significantly higher than in control rats, remaining within the normal range for this indicator. A moderate decrease in platelet levels in animals treated with NT with 0.3% silver may be due to the presence of anti-inflammatory activity of the sample. The urea concentration in the blood of animals after suturing NT with 0.5% silver was significantly lower than in control animals (p = 0.019), which may be due to an increase in silver concentration of the alloy and requires further study.

CONCLUSIONS: new materials based on titanium nickelide with the addition of silver do not have acute systemic or subacute toxicity.

Keywords: titanium nickelide, silver, experimental animals, toxicity.

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About the authors

Ivan I Gordienko

Ural State Medical University

Author for correspondence.
Email: ivan-gordienko@mail.ru
ORCID iD: 0000-0003-3157-4579
SPIN-code: 5368-0964
Scopus Author ID: 57198361838
ResearcherId: AEG-0529-2022

кандидат медицинских наук, доцент, доцент кафедры детской хирургии

Россия, 620028, Российская Федерация, Свердловская обл., г. Екатеринбург, ул. Репина, д. 3.

Maria N Dobrinskaya

Уральский государственный медицинский университет

Email: maria-nd@mail.ru
ORCID iD: 0000-0001-9208-9417

кандидат медицинских наук, доцент кафедры фармакологии и клинической фармакологии

Россия, 620028, Российская Федерация, Свердловская обл., г. Екатеринбург, ул. Репина, д. 3.

Sergey M Kutepov

Уральский государственный медицинский университет

Email: usma@usma.ru
ORCID iD: 0000-0002-3069-8150

член-корреспондент РАН, доктор медицинских наук, профессор, научный руководитель Института травматологии и ортопедии ЦНИЛ

Россия, 620028, Российская Федерация, Свердловская обл., г. Екатеринбург, ул. Репина, д. 3.

Nadezhda V Izmozherova

Уральский государственный медицинский университет

Email: nadezhda_izm@mail.ru
ORCID iD: 0000-0001-7826-9657

доктор медицинских наук, доцент, заведующий кафедрой фармакологии и клинической фармакологии

Россия, 620028, Российская Федерация, Свердловская обл., г. Екатеринбург, ул. Репина, д. 3.

Ekaterina S Marchenko

Национальный исследовательский Томский государственный университет

Email: 89138641814@mail.ru
ORCID iD: 0000-0003-4615-5270

доктор физико-математических наук, доцент, заведующий лабораторией медицинских сплавов и имплантатов с памятью формы

Россия

Irina P Antropova

Уральский государственный медицинский университет

Email: aip.hemolab@mail.ru
ORCID iD: 0000-0002-9957-2505

доктор биологических наук, ведущий научный сотрудник Института травматологии и ортопедии ЦНИЛ

Россия, 620028, Российская Федерация, Свердловская обл., г. Екатеринбург, ул. Репина, д. 3.

Larisa G Polushina

Уральский государственный медицинский университет

Email: polushina-larisa@bk.ru
ORCID iD: 0000-0002-4921-7222

кандидат медицинских наук, старший научный сотрудник ЦНИЛ

Россия, 620028, Российская Федерация, Свердловская обл., г. Екатеринбург, ул. Репина, д. 3.

Leonid P Larionov

Уральский государственный медицинский университет

Email: leonid-larionov@mail.ru

доктор медицинских наук, профессор, профессор кафедры фармакологии и клинической фармакологии

Россия, 620028, Российская Федерация, Свердловская обл., г. Екатеринбург, ул. Репина, д. 3.

Natalya A Tsap

Уральский государственный медицинский университет

Email: tsapna-ekat@rambler.ru
ORCID iD: 0000-0001-9050-3629

доктор медицинских наук, профессор, заслуженный врач Российской Федерации, заведующий кафедрой детской хирургии

Россия, 620028, Российская Федерация, Свердловская обл., г. Екатеринбург, ул. Репина, д. 3.

Stepan P Chernyii

Уральский государственный медицинский университет

Email: stechernyy@yandex.ru
ORCID iD: 0009-0002-0129-1244
SPIN-code: 2453-9105
Scopus Author ID: 1254222

ассистент кафедры детской хирургии, аспирант кафедры детской хирургии

Россия, 620028, Российская Федерация, Свердловская обл., г. Екатеринбург, ул. Репина, д. 3.

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Copyright (c) Gordienko I.I., Dobrinskaya M.N., Kutepov S.M., Izmozherova N.V., Marchenko E.S., Antropova I.P., Polushina L.G., Larionov L.P., Tsap N.A., Chernyii S.P.

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